Phenotypic and molecular traits diversity in soybean launched in forty years of genetic breeding

Mário do Carmo Oda; Tuneo Sediyama; Éder Matsuo; Cosme Damião Cruz; Everaldo Gonçalves de Barros; Marcia Flores da Silva Ferreira

doi:10.33158/ASB.2015v1i1p1

Authors

Mário do Carmo Oda Universidade Federal de Viçosa
Tuneo Sediyama Universidade Federal de Viçosa.
Éder Matsuo Universidade Federal de Viçosa
Cosme Damião Cruz Universidade Federal de Viçosa
Everaldo Gonçalves de Barros Universidade Federal de Viçosa
Marcia Flores da Silva Ferreira Universidade Federal do Espírito Santo

DOI:

https://doi.org/10.33158/ASB.2015v1i1p1

Keywords:

Glycine max, Germplasm, Genetic variability, SSR marker, Coefficient of parentage, Phenotypic characterization

Abstract

The aim of this study was to evaluate the diversity of phenotypic and molecular traits in soybean varieties launched in forty years of breeding. The DNA was amplified with 42 microsatellite markers (SSR). Polymorphism of 38 SSR markers were identified in polyacrylamide gel at 10%. 106 alleles were amplified with an average of 2.52 alleles per SSR locus. Polymorphism information content varied from 0 to 0.68 with an average of 0.38. Genetic dissimilarities between pairs of cultivars varied from 0.4 to 0.6, 0.8 to 1.0 and 0.0 to 0.4 for data btained from SSR markers, coefficient of parentage and phenotypic characters, respectively. It was possible to verify the contribution of cultivars considered old, intermediate and recent as well as the genetic variability of the group of cultivars used, which remained the same over 40 years of breeding. It was also observed that, with the combination of six microsatellite primers, it was possible to distinguish the 21 cultivars used in this study; and that microsatellite markers showed less biased estimates compared to the estimates obtained by the parentage coefficient and phenotypic characters in studies on genetic diversity.

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Author Biographies

Mário do Carmo Oda, Universidade Federal de Viçosa

Mestrado em andamento em Genética e Melhoramento
Tuneo Sediyama, Universidade Federal de Viçosa.

Pós-doutorado pela Purdue University. Professor titular da Universidade Federal de Viçosa.
Éder Matsuo, Universidade Federal de Viçosa

Doutorado em Genética e Melhoramento pela Universidade Federal de Viçosa. Professor na Universidade Federal de Viçosa -
Cosme Damião Cruz, Universidade Federal de Viçosa

Doutorado em Agronomia (Genética e Melhoramento de Plantas) pela Universidade de São Paulo
Everaldo Gonçalves de Barros, Universidade Federal de Viçosa

Doutorado em Purdue University em 1991. Professor titular aposentado pela Universidade Federal de Viçosa
Marcia Flores da Silva Ferreira, Universidade Federal do Espírito Santo

Doutorado em Genética e Melhoramento pela Universidade Federal de Viçosa

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