Collection periods in the in vitro establishment of raspberry tree cultivars

  • Cíntia de Moraes Fagundes Universidade Federal de Pelotas - UFPel
  • Roseane Maidana Moreira Universidade Federal de Pelotas - UFPel
  • Cari Rejane Fiss Timm Universidade Federal de Pelotas - UFPel
  • Jacqueline Barcelos da Silva Universidade Federal de Pelotas - UFPel
  • Luis Eduardo Corrêa Antunes Embrapa Clima Temperado
  • Marcia Wulff Schuch Universidade Federal de Pelotas - UFPel
Keywords: Tissues culture, Seasonality, Rubus idaeus, Phenolic oxidation

Abstract

The raspberry tree, a species from the Rosaceae family and the Rubus genus, is included in the group of small fruit that has been drawing the attention of the global consuming market for carrying antioxidant properties and substances capable of fighting free radicals, bringing health benefits. The objective of this work was to evaluate the effect of collection periods on the in vitro establishment of raspberry trees (Rubus idaeus L.). Explants were collected in different seasons of the year (winter, spring, summer and autumn), and were inoculated in a MS culture medium. The experimental design was totally randomized, and arranged in a factorial scheme. At 28 days, the following variables were evaluated: oxidation, fungal and bacterial contamination survival and establishment. There was less phenolic oxidation in raspberry tree explants collected in winter. Explants contamination by endogenic bacteria occurred in higher percentages in winter. Although summer was the season of the year with higher percentage of explants�?? phenolic oxidation for both cultivars, the highest in vitro establishment percentages for the Heritage cultivar occurred in summer and fall. However, for the Fallgold cultivar, spring and winter were the seasons with greater in vitroestablishment.

Downloads

Download data is not yet available.

Author Biographies

Cíntia de Moraes Fagundes, Universidade Federal de Pelotas - UFPel
Universidade Federal de Pelotas, Pós-Graduação em Agronomia, Avenida Eliseu Maciel, s/n, 96160-000, Pelotas, Rio Grande do Sul, Brazil.
Roseane Maidana Moreira, Universidade Federal de Pelotas - UFPel
Universidade Federal de Pelotas, Pós-Graduação em Agronomia, Avenida Eliseu Maciel, s/n, 96160-000, Pelotas, Rio Grande do Sul, Brazil.
Cari Rejane Fiss Timm, Universidade Federal de Pelotas - UFPel
Universidade Federal de Pelotas, Pós-Graduação em Agronomia, Avenida Eliseu Maciel, s/n, 96160-000, Pelotas, Rio Grande do Sul, Brazil.
Jacqueline Barcelos da Silva, Universidade Federal de Pelotas - UFPel
Universidade Federal de Pelotas, Pós-Graduação em Agronomia, Avenida Eliseu Maciel, s/n, 96160-000, Pelotas, Rio Grande do Sul, Brazil.
Luis Eduardo Corrêa Antunes, Embrapa Clima Temperado

Embrapa Clima Temperado, BR 392, km 78, 96010-971, 9º Distrito, Monte Bonito - Pelotas, Rio Grande do Sul, Brazil

Marcia Wulff Schuch, Universidade Federal de Pelotas - UFPel
Universidade Federal de Pelotas, Pós-Graduação em Agronomia, Avenida Eliseu Maciel, s/n, 96160-000, Pelotas, Rio Grande do Sul, Brazil.

References

Antoniolli LR, Silva GA, Alves SAM and MORO L (2011) Controle alternativo de podridões pós-colheita de framboesas. Pesquisa Agropecuária Brasileira 46(9): 979-985.

Buzkan N, Cetiner S and Yalcin-Mendi Y (1997) Clonal propagation of disease �?? free rootstocks for sour and sweet cherry by meristem culture. Acta Horticulturae 441: 329-332.

Chalfun NNJ and Pio R (2012) Aquisição e Plantio de Mudas Frutíferas. Boletim de Extensão. Editora UFLA, Lavras, MG, p. 8.

Erig AC and Fortes GRL (2002) Estabelecimento de pereira (Pyrus spp,) in vitro a partir de meristemas e gemas. Ciência Rural 32(4): 577-582.

Erig AC, Vicenzi M and Chaves AC (2003) Desinfestação de explantes de mirtilo (Vaccinium ashei Reade) visando o estabelecimento de plantas in vitro. Revista Científica Rural 8(1): 142-148.

Gonçalves ED, Pio R, Caproni CM, Zambon CR, Silva LFO and Alvarenga AA (2011) Implantação, cultivo e pós-colheita de framboesa no Sul de Minas Gerais. EPAMIG, Belo Horizonte, MG, p. 5. (Circular Técnica 145).

Han C, Zhao Y, Leonard SW and Traber MG (2007) Edible coatings to improve storability and enhance nutritional value of fresh an frozen strawberries (Fragaria X ananassa) and raspberries (Rubus idaeus). Postharvest Biology and Technology 33:67-78.

Ilha LLH (2012) Produção de amora-preta e framboesa em regiões de clima temperado. Informe Agropecuário, pequenas frutas: tecnologias de produção, 33(268): 58-68.

Karaklajic-Stajic Ž, Ružic D, Glišic IS and Lukovic J (2012) Vegetative pontential of plants of raspberry �??Willamette�?� grown on di_ erent growing media and treated with di_ erent foliar fertilizers. Acta Horticulturae, Proceedings 10thInternational Rubus and Ribes Symposium, 946: 391-396.

Kretzschmar AA, Rufato L, Pelizza TR and Ribeiro RS (2013) A cultura da framboeseira, UDESC, Florianópolis, p.194. (Série Fruticultura, 2).

Murashige T and Skoog F (1962) A revised medium for rapid growth and biossay with tabacco tissue cultures. Physiologia Plantarum 15: 473-497.

Oliveira RP, Rocha PSG, Gularte VF and Scivittaro WB (2010) Micropropagação de framboeseira em diferentes concentrações de ferro. Revista Ciência Rural 40(12): 2598-2602.

Raseira MC, Gonçalves EDG, Trevisan R, Antunes LEC (2004) Aspectos técnicos da cultura da framboeseira. Embrapa Clima Temperado, Pelotas, p. 22. (Technical Bulletin, 120).

Rosa FC, Hansel FA, Dutra LF and Quadros KM (2006) Micropropagação de erva-mate: efeito de diferentes épocas do ano no estabelecimento in vitro de segmentos nodais. Embrapa Florestas, p. 4. (Techinical Bulletin, 163).

Scherer-Salvaro LM, Echer MM, Guimaraes VF, Oglio EID and Fortes AMT (2009) �?poca de coleta dos explantes e fitotorreguladores na multiplicação in vitro de citronela de Java. Scientia Agraria 10: 267-274.

Schuch MW and Peil RMN (2011) Soilless cultivation systems: A new approach in fruit plants propagation in the south of Brazil, In: International Symposium on Advanced Technologies and management towards sustainable greenhouse ecosystems-Green Syszoll. Acta Horticulturae 952: 877-883.

Tezotto-Uliana JV and Kluge RA (2013) Framboesa: cultura alternativa para pequenas propriedades rurais em regiões subtropicais. ESALQ, Piracicaba, p.33. (Technical Bulletin, 55).

Toogod A (2007) Enciclopédia de la propagación de plantas. Blume, Barcelona, 320p.

Published
2017-11-30
How to Cite
Fagundes, C. de M., Moreira, R. M., Timm, C. R. F., Silva, J. B. da, Antunes, L. E. C., & Schuch, M. W. (2017). Collection periods in the in vitro establishment of raspberry tree cultivars. Agronomy Science and Biotechnology, 2(2), 92. https://doi.org/10.33158/ASB.2016v2i2p92
Section
Article

Most read articles by the same author(s)