Overexpression of full-length and partial DREB2A enhances soybean drought tolerance

Juliane Prela  Marinho; Renata Fuganti Pagliarini; Mayla Daiane Correa Molinari; Juliana Marcolino-Gomes; André Luís Hartmann  Caranhoto; Silvana Regina Rockenbach Marin; Maria Cristina Neves Oliveira; José Salvador Simonet Foloni; Carlos Lasaro Pereira Melo; Satoshi Kidokoro; Junya Mizoi; Norihito  Kanamori; Kazuko  Yamaguchi-Shinozaki; Kazuo  Nakashima; Alexandre Lima  Nepomuceno; Liliane Marcia Mertz-Henning

doi:10.33158/ASB.r141.v8.2022

Juliane Prela Marinho Embrapa Soja, Carlos João Strass Road. Orlando Amaral Acess. Warta. CEP 86001-970, Londrina, PR, Brazil.
Renata Fuganti Pagliarini Embrapa Soja, Carlos João Strass Road. Orlando Amaral Acess. Warta. CEP 86001-970, Londrina, PR, Brazil. https://orcid.org/0000-0001-9282-2826
Mayla Daiane Correa Molinari Embrapa Soja, Carlos João Strass Road. Orlando Amaral Acess. Warta. CEP 86001-970, Londrina, PR, Brazil. https://orcid.org/0000-0002-9135-0422
Juliana Marcolino-Gomes Embrapa Soja, Carlos João Strass Road. Orlando Amaral Acess. Warta. CEP 86001-970, Londrina, PR, Brazil.
André Luís Hartmann Caranhoto Embrapa Soja, Carlos João Strass Road. Orlando Amaral Acess. Warta. CEP 86001-970, Londrina, PR, Brazil. https://orcid.org/0000-0003-3209-7962
Silvana Regina Rockenbach Marin Embrapa Soja, Carlos João Strass Road. Orlando Amaral Acess. Warta. CEP 86001-970, Londrina, PR, Brazil.
Maria Cristina Neves Oliveira Embrapa Soja, Carlos João Strass Road. Orlando Amaral Acess. Warta. CEP 86001-970, Londrina, PR, Brazil.
José Salvador Simonet Foloni Embrapa Soja, Carlos João Strass Road. Orlando Amaral Acess. Warta. CEP 86001-970, Londrina, PR, Brazil.
Carlos Lasaro Pereira Melo Embrapa Soja, Carlos João Strass Road. Orlando Amaral Acess. Warta. CEP 86001-970, Londrina, PR, Brazil. https://orcid.org/0000-0003-4787-8382
Satoshi Kidokoro Laboratory of Plant Molecular Physiology. Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo, Japan.
Junya Mizoi Plant Molecular Physiology Laboratory. Applied Biological Chemistry Department. University of Tokyo, Bunkyo-ku, Tokyo, Japan. https://orcid.org/0000-0002-3383-8059
Norihito Kanamori Division of Biological Resources and Post-harvest. Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki, Japan.
Kazuko Yamaguchi-Shinozaki Plant Molecular Physiology Laboratory. Applied Biological Chemistry Department. University of Tokyo, Bunkyo-ku, Tokyo, Japan
Kazuo Nakashima Division of Biological Resources and Post-harvest. Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki, Japan. https://orcid.org/0000-0001-6417-8316
Alexandre Lima Nepomuceno Embrapa Soybean, Carlos João Strass Road. Orlando Amaral Acess. Warta. PO. Box 231 86001-970, Londrina, PR, Brazil.
Liliane Marcia Mertz-Henning Embrapa Soybean, Carlos João Strass Road. Orlando Amaral Acess. Warta. PO. Box 231 86001-970, Londrina, PR, Brazil. https://orcid.org/0000-0001-7622-0649

DOI: https://doi.org/10.33158/ASB.r141.v8.2022

Keywords: Glycine max, genetically modified plants, heat tolerance, activating responses to drought, silencing responses to drought, controlling responses to drought

Abstract

Soybean is an important commodity worldwide. Abiotic conditions can adversely disturb crop growth and final yield. The transcription factor Dehydration-Responsive Element-Binding Proteins 2 (DREB2) act as a regulator of drought-responses. This study aimed to characterize soybean plants genetically modified with GmDREB2A;2 FL and GmDREB2A;2 CA for molecular, physiological, and agronomic responses, at different developmental periods. Results showed that seedlings from GmDREB2A;2 FL event presented lower growth reduction under osmotic treatment during germination. The GmDREB2A;2 FL and GmDREB2A;2 CA events showed improved performance in experiments of water deficit imposed in the vegetative period and higher rates in physiological parameters. In the reproductive period, there was a trend of higher yield compounds in GM GmDREB2A;2 FL event when compared to other genotypes and treatments. It was suggested that GmDREB2A;2 FL event presented superior performance due to the higher expression levels of the cisgene and drought-induced genes.

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